Metabolism of D-Arabinose by Aerobacter aerogenes: Purification of the Isomerasea
نویسندگان
چکیده
enzyme was purified to homogeneity as indicated by a single band in disc-gel electrophoretic columns and single peaks with column chromatography and ultracentrifugation from the wild-type PRL-R3 strain, induced with L-fucose and two constitutive mutants, 502 and 510. The ratios of the activities of this isomerase on Darabinose and L-fucose remained constant throughout all purifications. The apparent Km of the isomerase from the wild-type strain induced with L-fucose and from the constitutive mutant strains was 5.0 x 10-2 M for L-fucose and 1.5 x 10-1
منابع مشابه
Metabolism of D-arabinose by Aerobacter aerogenes: purification of the isomerase.
In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. This enzyme was purified to homogeneity as indicated by a single band in disc-ge...
متن کاملMetabolism of Pentoses and Pentitols by Aerobacter Aerogenes. I. Demonstration of Pentose Isomerase, Pentulokinase, and Pentitol Dehydrogenase Enzyme Families.
Mortlock, R. P. (Michigan State University, East Lansing) and W. A. Wood. Metabolism of pentoses and pentitols by Aerobacter aerogenes. I. Demonstration of pentose isomerase, pentulokinase, and pentitol dehydrogenase enzyme families. J. Bacteriol. 88:838-844. 1964.-Aerobacter aerogenes PRL-R3 is capable of utilizing as sole substrates for energy and growth seven of the eight aldopentoses and al...
متن کاملRegulation of pentitol metabolism by Aerobacter aerogenes. I. Coordinate control of ribitol dehydrogenase and D-ribulokinase activities.
Induction studies on Aerobacter aerogenes strain PRL-R3, using ribitol as the inducer-substrate, indicated that two enzymes of ribitol catabolism, ribitol dehydrogenase and d-ribulokinase, are coordinately induced. The utilization of d-arabinose as a substrate resulted in the induction of ribitol dehydrogenase as well as d-ribulokinase. Mutants which were constitutive for ribitol dehydrogenase ...
متن کاملGrowth of Aerobacter aerogenes on D-arabinose and L-xylose.
Aerobacter aerogenes is noted for its versatility in being capable of growth by utilizing many of the rare and unnatural carbohydrates as substrates. The mechanism of growth on several of these unnatural carbohydrates has been previously reported. A. aerogenes PRL-R3 possesses the ability to synthesize, in response to the common substrate ribitol, a ribitol dehydrogenase which will also catalyz...
متن کاملPathway of L-xylose and L-lyxose degradation in Aerobacter aerogenes.
Pen&e utilization in Aerobacter aerogenes has been shown from fermentation studies with Cl%beled pentoses (1, 2) and with cell extracts (3) to involve the conversion of the pentose to n-xylulose 5-phosphate, which is further metabolized via n-sedoheptulose 7-phosphate, n-fructose 6-phosphate, and intermediates of the Embden-Meyerhof pathway. Although Aerobacter aerogenes can grow on all eight o...
متن کامل